FAQ PEIpro®
PEIpro® has been specifically developed for large-scale virus production (mostly AAV and LV but other viruses as well) in both adherent and suspension cells grown either in serum-containing or serum-free medium. PEIpro® is provided with extensive QCs making it suitable for therapeutic virus production.
PEIpro®, PEIpro®-HQ and PEIpro®-GMP are composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro.
PEIpro® is provided as an aqueous solution of linear PEI.
PEIpro® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 5°C ± 3°C to ensure long term stability.
Expiry date is indicated in the Certificate of Analysis available in “My Account”.
Depending on the type of viral vector, different subtypes of HEK-293 cells are preferred such as HEK-293 for adenovirus and AAV virus production, HEK-293T and HEK-293FT for AAV and lentivirus production. PEIpro® is suitable for virus production in both adherent and suspension subtypes of HEK-293 cells.
PEIpro® is compatible with various serum-containing media and commercially available synthetic media for virus production in both adherent and suspension HEK-293 cells (Table 1). For more information, please contact our Technical Support.
Different culture vessels including multilayers flasks such as HYPERFlasks™ and HYPERstacks™ (Corning), or fixed-bed culture systems such as iCELLis® bioreactors (Pall) are compatible with the use of PEIpro® in adherent HEK-293 cells. For virus production in suspension HEK-293 cells, large size shaker flasks or bioreactors can be used.
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