FAQ FectoVIR®-AAV

FectoVIR®-AAV is a transfection reagent used as raw material in AAV production process. FectoVIR®-AAV gives higher productivity than other commercially available transfection reagents and decreases global cost of production. Please find more information on the dedicated webpage: FectoVIR®-AAV (polyplus-sartorius.com).

The FectoVIR®-AAV structure is confidential and a patent has been filled by Polyplus. PEIpro®, FectoVIR®-AAV and FectoVIR®-LV are different both in terms of molecular structure and formulation. 

You will find starting conditions as well as a range for optimization in the dedicated protocol available in the My account. Polyplus offers a DoE (Design of Experiment) service to help you optimizing transfection conditions and gain time during process development. If you need further information, please contact our scientific support using ourcontact us form. 

 

 

Expiry date is indicated in the Certificate of Analysis available in ”My account”. 

We have successfully used FectoVIR®-AAV on suspension HEK-293 derivatives: HEK-293T, HEK-293F, Expi293, VPC, VPC2.0, etc... Customers confirmed that FectoVIR®-AAV works well in several commercial and proprietary suspension HEK-293 subclones. 

In house, we produce both AAV-2, AAV-5, AAV-8 and AAV-9. More serotypes have been tested by our customers, with comparable yield improvements. 

FectoVIR®-AAV shows a remarquable improvement in terms of viral titers in suspension and adherent systems. 

For lentivirus production, we recommend FectoVIR®-LV as it is currently the best product for this application, both in adherent and suspension cell culture systems. 

A residual test has been developed by Polyplus and transferred to selected service providers. Contact us for more information. 

FectoVIR®-AAV GMP is manufactured in compliance with the ICH Q7 GMP part II guidelines that ensure a validated and aseptic manufacturing process. In addition, FectoVIR®-AAV GMP is provided with a full regulatory documentation to support IND and ensure the full traceability of the product. Using pharmaceutical guideline to manufacture a transfection reagent will help you reduce the risk and ensure patient safety. 

FAQ FectoVIR®-LV

FectoVIR®-LV is the next generation of transfection reagent to produce lentiviral vectors used for gene modified cell therapies (CAR-T, CAR-NK, gene modified stem cell therapy etc..). It tooks 3 years to develop this new molecule and finally found a transfection solution that enables to increase LVV titers.

More details available : FectoVIR®-LV transfection reagent (polyplus-sartorius.com)

You will find starting conditions as well as a range for optimization in the dedicated protocol available in the ”My account”. Polyplus offers a DOE (Design of Experiment) service to help you optimizing transfection conditions and gain time during process development. If you need further information, please contact our scientific support using our contact us form.

Expiry date is indicated in the Certificate of Analysis available in ”My account”.

 

 

FectoVIR®-LV GMP is currently under development.

FAQ PEIpro®

PEIpro® has been specifically developed for large-scale virus production (mostly AAV and LV but other viruses as well) in both adherent and suspension cells grown either in serum-containing or serum-free medium. PEIpro® is provided with extensive QCs making it suitable for therapeutic virus production. 

PEIpro®, PEIpro®-HQ and PEIpro®-GMP are composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro.

PEIpro® is provided as an aqueous solution of linear PEI.

PEIpro® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 5°C ± 3°C to ensure long term stability. 

Expiry date is indicated in the Certificate of Analysis available in “My Account”. 

Depending on the type of viral vector, different subtypes of HEK-293 cells are preferred such as HEK-293 for adenovirus and AAV virus production, HEK-293T and HEK-293FT for AAV and lentivirus production. PEIpro®is suitable for virus production in both adherent and suspension subtypes of HEK-293 cells. 

PEIpro®is compatible with various serum-containing media and commercially available synthetic media for virus production in both adherent and suspension HEK-293 cells (Table 1). For more information, please contact our Technical Support. 

PEI - Virus yields compared with different culture media
Table 1: Virus yields using PEIpro® in different culture media for HEK-293 cells. 

Different culture vessels including multilayers flasks such as HYPERFlasks™ and HYPERstacks™ (Corning), or fixed-bed culture systems such as iCELLis® bioreactors (Pall) are compatible with the use of PEIpro® in adherent HEK-293 cells. For virus production in suspension HEK-293 cells, large size shaker flasks or bioreactors can be used. 

Need certificate of analysis ?

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus®Portal.

Create an account to discover our :

  • Certificate of Analysis
  • Plasmid Design Platform
  • Product Protocols
  • All exclusive gated content
  • e-Newsletter

Create Account Now

Other Informations

Transfection database

Search for publications in the Transfection Database with Polyplus transfection reagents or for transfection conditions.

Over 6000 publications, 1000 cell lines and primary cells available.

Product Documents

You have access to all the documents  transfection reagent.

In order to download a product protocol or a certificate of analysis, please create an account on Polyplus  

Get in touch with our

Scientific support specialists

48hr
For turn around
94%
Client Satisfaction
96%
Would use our service again

Your needs are too complex to be explained in few words ? Feel free to use our dedicated contact form for a full support !

Contact us

Do you have a question ?

Feel free to ask your question.Our team answer you quickly !