FectoVIR®-AAV is a transfection reagent used as raw material in AAV production process. FectoVIR®-AAV gives higher productivity than other commercially available transfection reagents and decreases global cost of production. Please find more information on the dedicated webpage: FectoVIR®-AAV (polyplus-sartorius.com).
You will find starting conditions as well as a range for optimization in the dedicated protocol available in the ”My account”. Polyplus offers a DoE (Design of Experiment) service to help you optimizing transfection conditions and gain time during process development. If you need further information, please contact our scientific support using our contact us form.
We have successfully used FectoVIR®-AAV on suspension HEK-293 derivatives: HEK-293T, HEK-293F, Expi293, VPC, VPC2.0, etc... Customers confirmed that FectoVIR®-AAV works well in several commercial and proprietary suspension HEK-293 subclones.
In house, we produce both AAV-2, AAV-5, AAV-8 and AAV-9. More serotypes have been tested by our customers, with comparable yield improvements.
FectoVIR®-AAV shows a remarquable improvement in terms of viral titers in suspension and adherent systems.
For lentivirus production, we recommend FectoVIR®-LV as it is currently the best product for this application, both in adherent and suspension cell culture systems.
A residual test has been developed by Polyplus and transferred to selected service providers. Contact us for more information.
FectoVIR®-AAV GMP is manufactured in compliance with the ICH Q7 GMP part II guidelines that ensure a validated and aseptic manufacturing process. In addition, FectoVIR®-AAV GMP is provided with a full regulatory documentation to support IND and ensure the full traceability of the product. Using pharmaceutical guideline to manufacture a transfection reagent will help you reduce the risk and ensure patient safety.
PEIpro® has been specifically developed for large-scale virus production (mostly AAV and LV but other viruses as well) in both adherent and suspension cells grown either in serum-containing or serum-free medium. PEIpro® is provided with extensive QCs making it suitable for therapeutic virus production.
PEIpro®, PEIpro®-HQ and PEIpro®-GMP are composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro.
PEIpro® is provided as an aqueous solution of linear PEI.
PEIpro® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 5°C ± 3°C to ensure long term stability.
Depending on the type of viral vector, different subtypes of HEK-293 cells are preferred such as HEK-293 for adenovirus and AAV virus production, HEK-293T and HEK-293FT for AAV and lentivirus production. PEIpro® is suitable for virus production in both adherent and suspension subtypes of HEK-293 cells.
Different culture vessels including multilayers flasks such as HYPERFlasks™ and HYPERstacks™ (Corning), or fixed-bed culture systems such as iCELLis® bioreactors (Pall) are compatible with the use of PEIpro® in adherent HEK-293 cells. For virus production in suspension HEK-293 cells, large size shaker flasks or bioreactors can be used.
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