FAQ: Protein production

FectoPRO® is the reagent of choice for biomanufacturing of proteins in suspension as it has been developed for scientists interested in obtaining best possible results with mammalian Transient Gene Expression systems. FectoPRO® ensures highest transfection efficiencies and protein yields in CHO and HEK-293 cell lines grown in variety of media. Moreover FectoPRO® shows great cell viability, reproducibility and consistency in results. FectoPRO® outperforms any other commercially available reagent and allows to optimize mammalian bioproduction processes (whatever scale used) as it requires equally less DNA and reagent.

For large or very-large scale experiments, you might want to consider PEIpro® as it is also very efficient in HEK-293 cells and cost-effective.

In the case of protein production in an adherent system, PEIpro® or jetOPTIMUS® may be considered.

FectoPRO® can be used without FectoPRO® booster, but it is recommended if you want to achieve high protein yields using low DNA amounts.

FectoPRO® works beautifully in regular and high cell density systems. FectoPRO® allows to reach higher yields in a shorter timeframe – No need to wait for 7 or 12 days of cell growth to produce recombinant proteins.

The optimal DNA amount, when used with the medium is 0.8 µg DNA per mL of cell culture as stated in the FectoCHO® Expression system protocol. If you use FectoPRO® with any other media refer to the FectoPRO® protocol for guidance. However, it should be noted that with the synergistic association of the FectoCHO™ CD Expression medium and the FectoPRO® powerful transfection reagent you will obtain better protein production yields.

For more informations and datas please visit our FectoCHO™ Expression system page.

Both FectoPRO® and PEIpro® hasve been shown to work beautifully with HEK293 cells in suspension and serum-free media. But it depends on what your application is: FectoPRO® is used for protein production whereas PEIpro® is used for virus production.

No, you don’t need to buy the medium first and the transfection reagent in a second time since the FectoCHO™ Expression system kit is perfectly suited to both adapt your cells to FectoCHO™ CD Expression medium and perform transfection using FectoPRO® transfection reagent. Indeed, in the FectoCHO™ Expression system kit (ref# 101000011), we provide you with 1L of medium and 1mL of transfection reagent, a perfect ratio of both reagents to allow you to conduct a full experiment from cell adaptation to protein production. Feel free to contact us for any pricing inquiry.

Depending on the medium used, 1 mL FectoPRO® is sufficient to transfect between 500mL and 1L of culture.

The DNA amount per mL is the same if you use either the  FectoCHO® expression system or the ExpiCHO™ expression system in ExpiCHO™ cells. However keep in mind that, with FectoCHO®, you can obtain up to twice the protein production yield at a lower cost.

FectoCHO® CD medium can be used for culture and transient protein expression with ExpiCHO® cells. However, it is important to note that it is not a plug and play system. A preliminary phase of adaptation and optimization is required.