Our fully integrated protein manufacturing services covers all needs from initial process development to manufacturing at research, HQ and GMP grade.
Meet our team at the 13th Conference on Protein Expression in Animal Cells (13th PEACe) in Valencia (Spain), September 24-28th, 2017.
Our solutions for Bioproduction
FectoPRO® Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
PEIpro® is a DNA transfection reagent for production of viruses, recombinant proteins and antibodies either in adherent cells in presence of serum or in suspension HEK-293 cells grown in low serum or serum-free media.
PEIpro®-HQ is a highly qualified grade of PEIpro® reagent, especially developed for the production of clinical batches of viral vectors or recombinant proteins and antibodies. The PEIpro®-HQ grade is supplied with appropriate Quality Controls and documentation allowing its use as a qualified raw material in GMP processes for the manufacturing of clinical batches of therapeutic products.
FectoPRO® : A novel and powerful way to obtain high protein yield production using Transient Gene Expression in CHO and HEK-293 cells using FectoPRO transfection reagent.
Transient protein expression is a common bioproduction process used to produce milligram to gram quantities of recombinant proteins and antibodies for a wide range of applications. However, when compared to stable expression platforms, the overall yield of transient protein expression remains weak, limiting its use in bioproduction processes. One of the manufacturing strategies for improving the transient productivity is then to increase transfection efficiency. We have developed a novel technologically advanced transfection solution, FectoPRO®, which outperforms currently available PEI-based and lipid-based transfection reagents. This poster presents data and protocol leading to remarkable protein and antibody yields in different transient CHO and HEK-293 expression systems. FectoPRO leads to higher transfection efficiency than any other currently available reagent and to very high protein yields. Transient transfection is now reproducible and easily scalable. In addition FectoPRO is compatiblewith a number of different media, including the Expi293 and ExpiCHO systems.
PEIpro®: High quality and Optimized PEI-based transfection reagents for production of clinical grade viral vectors.
Gene- and cell therapy-based medicines are experiencing resurgence due to the introduction of “next generation” transfer viral vectors, which have demonstrated improved safety and efficacy. Adeno Associated Virus (AAV) and Lentivirus are very commonly used in therapeutics and often produced using Polyethylenimine (PEI)-mediated transient transfection in HEK-293 or HEK-293T cells. The critical raw materials needed for cGMP vector production must be sourced from approved suppliers and should have gone through a rigorous testing program to reduce the risk of introducing adventitious agents into the production process. Polyplus-transfection now provides PEIpro® and its high-quality counterpart PEIpro®-HQ, the unique PEI-based transfection reagents suitable for use in process development and in cGMP biomanufacturing, respectively. Both reagents are particularly well suited for therapeutic virus production as they are PEI (Polyethylenime) based and free of components of animal-origin. They have been selected for their high transfection efficiency using low DNA amount. PEIpro® and PEIpro®-HQ undergo stringent quality controls for use in process development. In addition, impurity profile, residual organic solvent and heavy metal content are also tested in PEIpro®-HQ to assess the purity of the reagent. Moreover, each lot of PEIpro®-HQ is provided with an extensive documentation, making PEIpro®-HQ perfectly suitable for use as a qualified raw material for the production of clinical batches of viruses in GMP processes.
Location of the event
The convention will take place at Hotel Balneario Las Arenas:
Eugenia Viñes, 22-24. 46011, Valencia, Spain
For more information, please have a look at the agenda and register now to meet the delivery experts. Our team will present two Posters focusing on FectoPRO® & PEIpro®, our transfection reagent especially designed for Bioproduction.
Join us on the social network
We created a dedicated event on Facebook to allow you to discuss with other scientists about the PEACe meeting or about your transfection experiments.
If you want to talk about this event (Facebook, Twitter, Google+, etc…), don’t forget to use the #PEACe and follow the PEACe news.
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