Allergan (Abbvie) is a global pharmaceutical leader focused on developing, manufacturing and commercializing branded pharmaceutical, device, biologic, surgical and regenerative medicine products for patients around the world.

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Optimization of AAV process development: transfection matters

With the increased number of therapeutic rAAV candidates reaching the clinical trial pipeline, there is demand for innovative technologies to improve process development and facilitate manufacturing scale-up for future commercialization. To this end, Polyplus-transfection has worked hand-in-hand with viral vector manufacturers to develop a transfection reagent specifically for large scale manufacturing in suspension cell systems: FectoVIR®-AAV. FectoVIR®-AAV aims to improve rAAV manufacturing processes by boosting productivity, bringing flexibility and facilitating scalability. Here, we share preliminary data from Allergan Biologics’ recent evaluation of FectoVIR®-AAV against their current AAV production platform. Analysis of physical titers revealed a 3-fold increase in both viral particles (VP) and viral genome (VG) per ml of cell culture when using FectoVIR®-AAV transfection reagent compared to PEIpro®.