Citation
- Authors: Runz, S., Mierke, C. T., Joumaa, S., Behrens, J., Fabry, B., Altevogt, P.
- Year: 2008
- Journal: Biochem Biophys Res Commun 365 35-41
- Applications: in vitro / DNA / jetPEI
- Cell types:
- Name: A125
- Name: MDA-MB-435S
Description: Human breast cancer cells
Abstract
The expression of the glycosyl phosphatidylinositol (GPI)-anchored protein CD24 correlates with poor prognosis in a variety of carcinomas. However, little is known about the cellular mechanisms of the CD24-mediated effects. In this study, we present evidence that CD24 affects the lateral localization of beta1 integrin. Using stably CD24-transfected A125 and MDA-MB-435S carcinoma cells we show that CD24 augments beta1-dependent cell motility and stimulates transmigration and invasion across a monolayer of endothelial cells. Furthermore, as demonstrated by sucrose density gradient centrifugation and Western Blot analysis, CD24 recruits beta1 integrin into lipid raft domains. We suggest that CD24 acts as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins.