Citation
- Authors: Paul-Gilloteaux, P., Waharte, F., Singh, M. K., Parrini, M. C.
- Year: 2018
- Journal: Methods Mol Biol 1749 279-289
- Applications: in vitro / DNA / jetPRIME
- Cell type: HEK-HT
Description: Stably expressing the early region of SV40 (T-Ag) and the catalytic subunit of telomerase hTERT
Method
2 µg DNA + 4 µL jetPRIME in 3.5 cm dish
Abstract
During mesenchymal cell motility, various actin regulators are recruited to the leading edge with exquisite precision in time and space to generate protrusion and retraction cycles. We present here an automated method, named CorRecD (from Correlation Recruitment Dynamics), which quantifies cell edge dynamics, protein recruitment and analyze their cross-correlation. The Wave Regulatory Complex (WRC), a master driver of protrusions, is used as a case-of-study. This biologist-friendly method relies on free software tools and can be applied to any fluorescently tagged protein of interest.