FectoPRO is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells.
Meet our Team by visiting our virtual booth and listen to our talk during The Bioprocessing Summit Europe !
A next-generation transfection reagent for large scale rAAV manufacturing.
The number of ATMP therapeutic-based medicines for inherited genetic disorders is in constant growth, with a global 32% increase in new clinical trials in the last 4 years. ATMPs have demonstrated their success with already more than ten approved for commercialization. The success of AAV as the most promising viral vector for gene therapy is due to low immunogenicity, broad tropism and non-integrating properties. One major challenge for translation of promising research to clinical development is the manufacture of sufficient quantities of AAV. Transient transfection of suspension cells is the most commonly used production platform, as it offers significant flexibility for cell and gene therapy development. However, this method shows some limitations in large scale bioreactors: inadequate transfection protocol, reduced transfection efficiency and lower productivity. To address this concern, we present data on the novel transfection reagent showing: i) increased AAV titers, ii) improved transfection protocol for large scale bioreactors and iii) reproducibility of viral titers at different production scale. The aforementioned optimized parameters make this novel transfection reagent ideal for cell and gene therapy developers by combining the flexibility of transient transfection with scalability and speed to market.
Presented by Mégane Denu, Research Engineer in Bioprodcution at Polyplus-transfection®
Wednesday, March 17th at 11:25 am – 11:45 am CET
Next-Generation Transfection Reagent for Large Scale AAV Manufacturing
Mathieu Porte, Mégane Denu, Jonathan Havard, Marine Ricordel, Coralie Stritt, Yann Philipson, Malik Hellal, Patrick Erbacher