Our team

 Mathieu Porte 

 Bioproduction R&D Manager  

 Brian Bulsak 

 Sales Specialist - Mid-Atlantic 

 Geraldine Guerin-Peyrou 

 Marketing & Communication Director 

 Tom KRESGE 

 VP Sales US 

Talk: Next-Generation Transfection Reagent for Large Scale AAV Manufacturing

The number of ATMP therapeutic-based medicines for inherited genetic disorders is in constant growth, with a global 32% increase in new clinical trials in the last 4 years. ATMPs have demonstrated their success with already more than ten approved for commercialization. The success of AAV as the most promising viral vector for gene therapy is due to low immunogenicity, broad tropism and non-integrating properties. One major challenge for translation of promising research to clinical development is the manufacture of sufficient quantities of AAV.

Transient transfection of suspension cells is the most commonly used production platform, as it offers significant flexibility for cell and gene therapy development. However, this method presents some limitations in large scale bioreactors: inadequate transfection protocol, reduced transfection efficiency and lower productivity. To address this concern, we present data on a novel transfection reagent showing: I) increased AAV titers, II) improved transfection protocol for large scale bioreactors, III) reproducibility of viral titers at different production scale and IV) customer data. The aforementioned optimized parameters make this novel transfection reagent ideal for cell and gene therapy developers by combining the flexibility of transient transfection with scalability and speed to market.

Presented by Mathieu PORTE, R&D Bioproduction Manager at Polyplus^®

Monday May 16^th from 3:45 pm – 4:00 pm ET at Tools and Technology Forum.

Poster

Next-Generation Transfection Reagent for Large Scale AAV Manufacturing

Mathieu Porte, Mégane Denu, Jonathan Havard, Marine Ricordel, Coralie Stritt, Yann Philipson, Malik Hellal, Patrick Erbacher