Our team

 Younes BOULILA 

 Business Manager 

 Jean-Thomas ISSENHUTH 

 Key Account Manager 

 Nadia Mazzaro 

 Scientific Support Specialist 

 Yohann FAIVRE 

  

Poster

Improving transient CHO and HEK-293 Expression Systems with a powerful transfection solution for high protein production yields: FectoPRO^®

Development process for biotherapeutic protein production usually begins with generating a high-performing stable cell line which can be used for manufacturing. As this step takes a lot of time, transient transfection offers a great alternative to quickly produce milligram to gram quantities of recombinant proteins and antibodies. A various number of culture media are available for performing transient protein production in both CHO and HEK cells, but the limiting factor often remains the transfection reagent. Therefore, Polyplus has developed a novel technologically advanced transfection solution named FectoPRO^®. Here we show that FectoPRO^® outperforms currently available PEI-based and lipid-based transfection reagents in all the transient expression systems tested, offering great transfection efficiency and amazing protein yields.