Protocol
- Description: Primary human hepatocytes
- Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
| Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | Hepatocyte Maint. Media w/ Supplement (ref: MM250, Lonza) |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | Forward protocol: 40 000 cells/ well |
| Transfection volume | 150 µL |
| Amount of biomolecule | 30 nM |
| Amount of reagent | 0.3 µL |
| Transfection analysis performed after | 48 h |