Protocol
- Description: Human prostate carcinoma cells
Known as: PC3, PC 3 - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Coon's modified Ham's F12 + Kaign's modified Ham's F12, 45 mg/L ascorbic acid, 7% FBS, 2 mM glutamine, 18 mg/L Inositol |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 30 000 cells per well |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 10 nM |
| Amount of reagent | 1 µl |
| Transfection analysis performed after | 48 h |