Protocol
- Description: Murine embryonic fibroblasts
Known as: NIH/3T3, 3T3 - Delivered Biomolecule: siRNA
| Transfection Reagent | jetPRIME |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM containing 4.5 g/l glucose, 2 mM glutamine, antibiotics, 10% New born calf serum |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 25 000 cells per well |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 20 nM |
| Amount of reagent | 2 µl |
| Transfection analysis performed after | 48 h |