Protocol

• Description: Human lung fibroblast cells
  Known as: MRC5, MRC 5
• Delivered Biomolecule: siRNA

Transfection Reagent	INTERFERin
Plate format	24-well plate
Cell culture conditions	MEM with Earle's salt, 2 mM glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, antibiotics, 10% FBS
Transfection medium	Same as above
Number of seeded cells/Confluency	30 - 50%
Transfection volume	0.5 ml
Amount of biomolecule	10 nM
Amount of reagent	1 µl
Transfection analysis performed after	48 h