Protocol

• Description: Canine kidney epithelial cells
• Delivered Biomolecule: DNA

Transfection Reagent	jetOPTIMUS
Plate format	24-well plate
Cell culture conditions	EMEM + 10 FBS% + 2% Pen/Strep + 1% Glutamine
Transfection medium	Complete Medium followed by a medium change 4h post-transfection with complete medium
Number of seeded cells/Confluency	40 000 cells
Transfection volume	500 µL
Amount of biomolecule	500 ng
Amount of reagent	0.5 µL
Transfection analysis performed after	24h