Protocol
- Description: Human breast adenocarcinoma cells
Known as: MDAMB231 - Delivered Biomolecule: DNA
| Transfection Reagent | jetPRIME |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM 4.5g/l glucose (high glucose) , 1% AANE, 10% FBS, 1% Glu, 1% Pen / Strep Either incubation of the complexes during 30 min or incubation of the complexes during 10 min + centrifugation of the plate 5 min at 180g just after the transfection |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 50 000 cells/well |
| Transfection volume | 0,5 ml |
| Amount of biomolecule | 0.75 µg |
| Amount of reagent | 1.5 µl |
| Transfection analysis performed after | 24 h |