Protocol
- Description: Human breast adenocarcinoma cells
Known as: MCF10A, MCF 10A - Delivered Biomolecule: DNA
| Transfection Reagent | jetPRIME |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Incubation of the complexes during 15 minutes; centrifuge the plate 5 min at 180g just after the transfection |
| Transfection medium | not specified |
| Number of seeded cells/Confluency | 60 000 cells/well |
| Transfection volume | not specified |
| Amount of biomolecule | 0.75 µg |
| Amount of reagent | 1.5 µl |
| Transfection analysis performed after | 24 h |