Protocol

  • Description: Human chronic myelogenous leukaemia cells
    Known as: K562
  • Delivered Biomolecule: siRNA
Transfection Reagent INTERFERin
Plate format 24-well plate
Cell culture conditions RPMI with 2 mM glutamine, antibiotics, 10% FBS
Transfection medium Same as above
Number of seeded cells/Confluency not specified
Transfection volume 0.3 ml
Amount of biomolecule 50 nM
Amount of reagent 3 µl
Transfection analysis performed after 48 h