Protocol
- Description: Human acute T cell leukemia line
- Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | RPMI with 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 1x105 cells per well |
| Transfection volume | 1 ml |
| Amount of biomolecule | 2 µg |
| Amount of reagent | 6.4 µl |
| Transfection analysis performed after | 24 h |