Protocol
- Description: Human umbilical vein endothelial cells
- Delivered Biomolecule: DNA
| Transfection Reagent | jetOPTIMUS |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Endothelial cell growth medium + Supplement mix (Fetal calf serum, Endothelial cell growth supplement, Epidermal Growth FactorBasic Fibroblast Growth Factor, Heparin, Hydrocortisone) |
| Transfection medium | Basic Medium followed by a medium change 4h post-transfection with complete medium |
| Number of seeded cells/Confluency | 20 000 cells |
| Transfection volume | 500 µL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 0.75 µL |
| Transfection analysis performed after | 24h |