Protocol
- Description: Primary human fibroblast cells
- Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Fibroblast medium basal, FBS, Insulin, rhFGF, antibiotics |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 30 - 50% |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 1 nM |
| Amount of reagent | 2 µl |
| Transfection analysis performed after | 48 h |