Protocol

  • Description: Human cervix epitheloid carcinoma cells
  • Delivered Biomolecule: DNA
Transfection Reagent jetPRIME
Plate format 24-well plate
Cell culture conditions MEM with Earle's salt, 2 mM glutamine, antibiotics, 10% FBS
Transfection medium Same as above
Number of seeded cells/Confluency 50 000 cells per well
Transfection volume 0.5 ml
Amount of biomolecule 0.25 µg
Amount of reagent 0.5 µL
Transfection analysis performed after 24 h