Protocol
- Description: Human colon carcinoma cells
Known as: HCT116 - Delivered Biomolecule: DNA
| Transfection Reagent | jetPRIME |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM with 4.5 g/L glucose, 50 mM Hepes, 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 50 000 cells per well |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 0.5 µg |
| Amount of reagent | 1.5 µl |
| Transfection analysis performed after | 24 h |