Protocol

• Description: African green monkey kidney cells
  Known as: COS, COS7
• Delivered Biomolecule: DNA

Transfection Reagent	jetPRIME
Plate format	24-well plate
Cell culture conditions	DMEM with 4.5 g/L glucose, 50 mM Hepes, 2 mM glutamine, antibiotics, 10% FBS
Transfection medium	Same as above
Number of seeded cells/Confluency	40 000 cells per well
Transfection volume	0.5 ml
Amount of biomolecule	0.5 µg
Amount of reagent	1 µl
Transfection analysis performed after	24 h