Protocol

  • Description: Chinese hamster ovary cells
  • Delivered Biomolecule: DNA
Transfection Reagent FectoPRO, FectoCHO Expression System
Plate format shaker flask
Cell culture conditions

FectoCHO® CD Medium (Polyplus-transfection®), FreeStyle™ F17 (Life Technologies™), CHO-S SFM-II (Life Technologies™), CD-FortiCHO™ (Life Technologies™), FreeStyle™ CHO (Life Technologies™), ProCHO™ 4
(Lonza®), CD-CHO™ (Life Technologies™), HyClone™ HyCell™ TransFx™-C (GE Healthcare)
Transfection medium Same as above
Number of seeded cells/Confluency 1 x 10e6/ ml one day before transfection
Transfection volume Contact Tech Support for more details
Amount of biomolecule Contact Tech Support for more details
Amount of reagent Contact Tech Support for more details
Transfection analysis performed after -