Protocol
- Description: Chinese hamster ovary cells
- Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | HAM F12 with 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 10 000 cells per well |
| Transfection volume | 125 µl |
| Amount of biomolecule | 10 nM |
| Amount of reagent | 0.75 µl |
| Transfection analysis performed after | 48 h |