Citation

  • Authors: Poudyal, D., Cui, X., Le, P. M., Hofseth, A. B., Windust, A., Nagarkatti, M., Nagarkatti, P. S., Schetter, A. J., Harris, C. C., Hofseth, L. J.
  • Year: 2013
  • Journal: PLoS ONE 8 e75034
  • Applications: in vitro / antimiR, siRNA / INTERFERin
  • Cell types:
    1. Name: DLD-1
      Description: Human colorectal adenocarcinoma 
      Known as: DLD1, DLD 1
    2. Name: HCT 116
      Description: Human colon carcinoma cells
      Known as: HCT116
    3. Name: LoVo
      Description: Human colorectal adenocarcinoma cells

Method

0.1-10 nM ( siRNA), 10-50 nM (antimiR)

Abstract

Metastasis of colon cancer cells increases the risk of colon cancer mortality. We have recently shown that American ginseng prevents colon cancer, and a Hexane extract of American Ginseng (HAG) has particularly potent anti-inflammatory and anti-cancer properties. Dysregulated microRNA (miR) expression has been observed in several disease conditions including colon cancer. Using global miR expression profiling, we observed increased miR-29b in colon cancer cells following exposure to HAG. Since miR-29b plays a role in regulating the migration of cancer cells, we hypothesized that HAG induces miR-29b expression to target matrix metalloproteinase-2 (MMP-2) thereby suppressing the migration of colon cancer cells. Results are consistent with this hypothesis. Our study supports the understanding that targeting MMP-2 by miR-29b is a mechanism by which HAG suppresses the migration of colon cancer cells.

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