PULSin®

Specifications
Overview
Capabilities
All Data
Resources

Specifications

Reagent	PULSin^®
Molecule delivered	Protein; Antibody; Peptide
Cell types	Adherent & suspension cells grown in presence of serum
Number of transfection	0.4 ml of PULSin^® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
Storage	Store PULSin^® at 5 °C ± 3°C. Do not freeze. Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.
Provided with	Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control

Overview

The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.

PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.

Ordering Information

Reference number	Amount of reagent	Positive control (R-phycoerythrin)	Hepes dilution buffer
101000010	PULSin® 0.4 mL	20 µg	20 ml
501-04	0.4 ml	20 µg	20 ml

0.4 ml of PULSin^® protein delivery reagent is sufficient for 25 delivery experiment in 6-well plates.
For bulk quantities, please contact us.

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Capabilities

PULSin® enables delivery of protein irrespective of its size

PULSin® is able to deliver R-phycoerythrin, a fluorescent protein (240 kD), to the cytoplasm of up to 98% cells. The protein is evenly distributed in the cytoplasm and excluded from the nucleus due to its large size.

PULSin® efficiently delivers antibodies in live cells

Antibodies are successfully delivered in mammalian cells and able to recognize their target protein inside the cytoplasm. PULSin® enabled the delivery of FITC-labeled anti-alpha-tubulin to the cytoplasm of high percentage of HeLa cells (85%). Similarly, anti-giantin Alexa Fluor® 488 was delivered to the cytoplasm of 98% of live HeLa cells, labeling the Golgi apparatus.

Simple protocol for successful protein delivery.

PULSin® will save you time and efforts compared to other techniques using viral transduction or chemical conjugation. PULSin® reagent is ready-to-use and provided with a dilution buffer and a fluorescent control protein (R-phycoerythrin).
The protocol is fast: simply add the reagent to the protein, incubate and add to the cells. Cells can be analyzed starting 4 hours post-delivery.

Easy to use alternative to chemical conjugation and viral transduction.

Comparison of PULSin® with two other protein delivery reagents shows a higher efficiency of protein delivery, Moreover, the amount of protein delivered per cell is higher with PULSin® as measured for R-phycoerythrin protein and for FITC-alpha-tubulin antibody (cf additional data).